Potensi Pemuliaan Mutasi Radiasi sebagai upaya Peningkatan Variasi Genetik pada Tanaman Hias

Fitri Damayanti(1*)

(1) Universitas Indraprasta PGRI
(*) Corresponding Author

Abstract


Pemuliaan tanaman berbasis induksi mutasi radiasi memiliki peranan penting untuk peningkatan kualitas atau daya hasil tinggi pada tanaman hias. Induksi mutasi berarti perbaikan mutu genetik untuk memperoleh tanaman dengan sifat-sifat yang diinginkan melalui perubahan susunan genetik suatu tanaman. Secara komersial, tanaman hias yang diminati adalah memiliki variasi genetik yang tinggi seperti bentuk dan corak daun, warna bunga, masa penyimpanan bunga yang panjang, dan tahan terhadap hama dan penyakit. Teknologi induksi mutasi radiasi yang dilakukan melalui kultur jaringan terbukti mampu menghasilkan sejumlah besar mutan, yaitu dihasilkannya variasi genetik yang berbeda dengan tanaman liarnya. Keuntungan aplikasi induksi mutasi yang dikombinasi dengan teknik kultur jaringan adalah mutasi terjadi dapat timbul pada tingkat sel. Hal ini berpeluang tinggi untuk mendapatkan variasi genetik lebih besar dari teknik konvensional. Keberhasilan kegiatan pemuliaan melalui teknik mutasi radiasi dipengaruhi oleh bagian tanaman yang diaplikasikan dan konsentrasi dosis radiasi yang efektif. Tulisan ini adalah ulasan literatur mengenai potensi dan aplikasi teknik pemuliaan mutasi radiasi yang berfokus pada dosis radiasi sinar gamma yang efektif terhadap induksi karakteristik kebaharuan dari tanaman hias.


Keywords


Kultur jaringan, Pemuliaan tanaman, Sinar Gamma, Variasi genetik

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References


Ahloowalia, B. S., & Maluszynski, M. (2001). Induced mutations-a new paradigm in plant breeding. Euphytica, 118, 167-173. https://doi.org/10.1023/A:1004162323428.

Billore, V., Mirajkar, S. J., Suprasanna, P., & Jain, M. (2019). Gamma irradiation induced effects on in vitro shoot cultures and influence of monochromatic light regimes on irradiated shoot cultures of Dendrobium sonia orchid. Biotechnology Reports, 22, 1-7.

https://doi.org/10.1016/j.btre.2019.e00343

Dahab, A. M. A., Heikal, A. A. M., Taha, L. S., Gabr, A. M. M., Metwally, S. A., & Ali, A. A. R. (2017). In vitro mutagenesis induction in Eustoma grandiflorum plant using gamma radiation. Journal of Environmental Science and Technology, 10(4), 175–185. https://doi.org/10.3923/jest.2017.175.185.

Damayanti, F., Roostika, I., & Mansur, M. (2011). Induksi Keragaman Somaklonal pada Tunas Kantong Semar dengan Radiasi Sinar Gamma secara In Vitro. In Seminar Nasional Sains dan Teknologi Nuklir (pp. 276-281). Bandung: Indonesia: Batan dan UPI.

Dehgahi, R., & Joniyasa, A. (2017). Gamma irradiation induced variation in Dendrobium sonia-28 orchid protocorm-like bodies (PLBs). Fungal Genom Biol, 7, 1-11. https://doi.org/10.4172/ 2165-8056.1000151.

Diaz, L. E., García, S. A. L., Morales, R. A., Baez, R. I., Perez, V. E., Olivar, H. A., & Loeza, C. J. M. (2018). Effect of gamma radiation of 60Co on sunflower plants (Helianthus annuus L.) (Asteraceae), from irradiated achenes. Sci Agropecu, 9, 313-317. https://doi.org/10.17268/sci.agropecu.2018.03.02.

Duncan, D. R., & Widholm, J. M. (1990). Techniques for Selecting Mutan from Plant Tissue Culture. In: Pollaer, J. W., & Walker, J. M. (Eds.) Plant Cell, Tissue Culture: Methods in Molecular Biology (pp. 443-465). 6th. The Human Press: New York.

El-Khateeb, M. A., Rawia, A., Eid-Heba, A., Mahfouze., Ashor, H. A., & Mabrouk, R. M. S. (2017). Induction of mutation with gamma radiation in Helichrysum bracteatum L. and identification of mutants by molecular markers. Middle East Journal of Agriculture Research, 6(2), 282–293.

Ghani, M., & Sharma, S. K. (2019). Induction of powdery mildew resistance in gerbera (Gerbera jamesonii) through gamma irradiation. Physiol Mol Biol Plants, 25, 159-166. https://doi.org/10.1007/s12298-018-0613-5.

Hameed, A., Shah, T. M., Atta, B. M., Haq, M. A., & Sayed, H. (2008). Gamma irradiation effects on seed germination and growth, protein content, peroxidase and protease activity, lipid peroxidation in desi and kabuli chickpea. Pak. J. Bot, 40(3), 1033-1041.

Hendratno., & Mugiono. (1996). Present Status of Plant Mutation Breeding in Indonesia: Plant Mutation Breeding in Asia. In International Conference of Plant Mutation Breeding (pp. 21-37). Beijing, China.

Khatab, I. A., & Hegazi, M. A. (2015). Induction of genetic variability with gamma radiation in some flowering ornamental herbs. International Journal of Current Research in Biosciences and Plant Biology, 2(6), 88–93.

Kim, J. H., Baek, M. H., Chung, B. Y., Wi S. G., & Kim, J. S. (2004). Alterations in the photosynthetic pigments and antioxidant machineries of red pepper (Capsicum annuum L.) seedlings from gamma irradiated seeds. J. Plant Biol, 47, 314-321.

Kim, S. H., Kim, S. W., Ahn, J. W., Ryu, J., Kwon, S. J., Kang, B. C., & Kim, J. B. (2020). Frequency, spectrum, and stability of leaf mutants induced by diverse ?-ray treatments in two Cymbidium hybrids. Plants, 9(4), 1-11. https://doi.org/10.3390/plants9040546.

Kodym, A., & Afza, R. (2003). Physical and chemical mutagenesis. Methods Mol Biol, 236, 189-204. https://doi.org/10.1385/1-59259- 413-1:189.

Lagoda, P. J. L. (2012). Effects of Radiation on Living Cells and Plants. In: Shu, Q. Y., Forster, B. P., & Nakagawa, H. (Eds.) Plant Mutation Breeding and Biotechnology (pp. 108-134). CAB e-Book. https://doi.org/10.1079/9781780640853.0123.

Larkin, P. J., & Scowcroft, W. R. (1981). Somaclonal variation annovel source of variability from cell culture for plant improvement. Theor. Appl. Genet, 60, 197-214.

Maity, J. P., Mishra, D., Chakraborty, A., Saha, A., Santra, S. C., & Chanda, S. (2005). Modulation of some quantitative and qualitative characteristics in rice (Oryza sativa L.) and mung (Phaseolus mungo L.) by ionizing radiation. Radiat Phys Chem, 74, 391-394. https://doi.org/10.1016/j.radphyschem.2004.08.005.

Majeed, A., Muhammad, Z., Ullah, R., Ullah, Z., Ullah, R., Chaudhry, Z., & Siyar, S. (2017). Effect of gamma irradiation on growth and post-harvest storage of vegetables. PSM Biol Res, 2, 30-35.

Majeed, A., Muhammad, Z., Ullah, R., & Ali, H. (2018). Gamma irradiation in: effect on germination and general growth characteristics of plants-A review. Pak J Bot, 50, 2449-2453.

Muhallilin, I., Aisyah, S. I., & Sukma, D. (2019). The diversity of morphological characteristics and chemical content of Celosia cristata plantlets due to gamma ray irradiation. Biodiversitas, 20(3), 862–866. https://doi.org/10.13057/biodiv/d200333.

Patil, U. H., Karale, A. R., Katwate, S. M., & Patil, M. S. (2017). Mutation breeding in chrysanthemum (Dendranthema grandiflora T.). J Pharmacogn Phytochem, 6, 230-232.

Piri, I., Babayan, M., Tavassoli, A., & Javaheri, M. (2011). The use of gamma irradiation in agriculture. Afr J Microbiol Res, 5, 5806-5811. https://doi.org/10.5897/AJMR11.949.

Saputra, G. A. (2020). Monstera adansonii Variegata, Tanaman Hit Harga Selangit. https://mediaindonesia.com/read/detail/328485-monstera-adansonii-variegata-tanaman-hit-harga-selangit. Diakses tanggal 1 Oktober 2020.

Setia, M. K., Bala, M., & Singh, S. (2020). Induction of novel inflorescence traits in Chrysanthemum through 60Co gamma irradiation. International Journal of Radiation Biology, 96(10), 1309–1316. https://doi.org/10.1080/09553002.2020.1793023.

Shu, Q. Y., Forster, B. P., & Nakagawa, H. (2012). Plant Mutation Breeding and Biotechnology. In: Kharkwal, M. C. (Ed). A Brief History of Plant Mutagenesis (pp 21-30). CABI. https://doi.org/10.1079/9781780640853.0021.

Shukla, A., Kashyap, S., Ramteke, V., Sinha, L., & Netam, M. (2018). Effect of gamma rays on flowering and vase life of gladiolus (Gladiolus grandiflorus L.). J of Pharmacognosy and Phytochemistry, 7, 558-561.

Spencer-Lopes, M. M., Forster, B. P., & Jankuloski, L. (2018). Manual on Mutation Breeding. 3rd. Food and Agriculture Organization of the United Nations (FAO).

Wi, S. G., Chung, B. Y., Kim, J. H., Baek, M. H., Yang, D. H., Lee, J. W., & Kim, J. S. (2005). Ultrastructural changes of cell organelles in Arabidopsis stem after gamma irradiation. J. Plant Biol, 482, 195-200.

Yufdy, M. P., Soedarjo, M., Marwoto, B., Winarto, B., Rianawati, S., Setyowati. A. S., Rahardjo, I. B., Djatnika, I., Tasman, E., Saefulloh, A., Badriah, D. S., & Sulyo, Y. (2012). Revitalisasi Balai Penelitian Tanaman Hias Mendukung Peningkatan Kualitas dan Daya Saing Produk Florikultura. Balithi: Jakarta.

Van den Bulk, R. W. (1991). Application of cell and tissue culture and in vitro selection for disease resistance breeding–a review. Euphytica, 56, 269-285.




DOI: http://dx.doi.org/10.30998/edubiologia.v1i2.9300

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